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叛纤酪屠 菟攀倚瓮屠 寥了任遗世 - 棱蝾疱翦疣螓, 滂耨屦蜞鲨, 觐眙屦屙鲨
 


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蕾纫 菔严信蜒热 门遗形宋萌淄壅 门臀 倚劳衙磐哇 欣岩磐冗 衔研拍岩挛 耐-衔阉拍温酪潘芡窝遗, 遗刑韧刃愚偃 倚劳咽腥现绒. 蔫耨屦蜞鲨 磬 耦桉赅龛 篦屙铋 耱屣屙 赅礓桎囹 徼铍钽梓 ...

-- [ 羊疣龛鲟 4 ] --

沅 囫耔祆弪痂 聆羼, m A mE 桴 铠栳觇 疱镳彗屙蜞蜩忭铖蜩. 项耠 镱漶蜞眍怅 珥圜屙栝 礤 漕腈眍 铌噻囹 蜞, 黩钺 囫耔祆弪痂 聆羼 镳邂嚯 犷脲 麇 怛痤 疋铊 铠栳觇 疱镳彗屙蜞蜩忭铖蜩, 镳铗桠眍 耠篦噱 疣耧疱溴脲龛 礤朦 聍栩囹 眍痨嚯. 礼耔戾蝠棹 聆羼 疣耨麒螓忄弪 镱 祛潴膻. 亦 驽 镳铊玮钿桦 镱漶麇 滂耧屦耔 囫耔戾蝠梃 (Da) 聆羼襦 (De) Da= (6*(n-1))/((n+1)*(n+3)) De = (24*n*(n-2)*(n-3))/((n+1)*(n+1)*(n+3)*(n+5)) 腮屐 耦铗眍龛 3*饰信蛙(Da) 栻孱疱蜩麇耜 囫耔戾蝠 5*饰信蛙(De)栻孱疱蜩麇耜栝 聆羼

橡铊玮钿桁 耦镱耱噔脲龛 忮腓麒:

|A| = 3*饰信蛙(Da) |E| = 5*饰信蛙(De) 橡 耦犭屙梃 钽 蝠遽钼囗, 疣耧疱溴脲龛 祛骓 聍栩囹 眍痨嚯. 埋 镱塍麇眄 疱珞朦蜞蝈 溧眄 镳邃耱噔脲睇 蜞犭桷 5.

如 蜞犭桷 5 忤漤, 黩 潆 怦艴 镱矬鲨 铒桉囗睇 恹 蝠遽钼囗 耦犭屙. 袜镳桁屦, 潆 耔眚弪梓羼觇 屐屙蝾 |A|=0,133595, 黩 戾睃 麇 3*饰信蛙=0,65709, |E|=0,40403, 戾睃 麇 5*饰信蛙=2,101393.

亦犭桷 5. 朽聍弪 囫耔祆弪痂 聆羼襦.

   

0,133595 -0,32106 -0,072463 0,189716 0,249603 -0,40403 0,206536 0,439709 0,175867 -0,31314

   

令脲 蝾泐, 潆 镳钼屦觇 汨镱蝈琨 耦铗忮蝰蜮 疣耧疱溴脲龛 眍痨嚯铎 桉镱朦珞屐 蜞 驽 钿桧 桤 溻篚 牮栩屦桢: 腓犷 暑腱钽铕钼嘌扈痦钼 腓犷 剜镨痤-予腙. 橡噔桦, 镱忤怿邋 疱珞朦蜞蝈 耩噔礤龛 忮痤蝽铖蝈 铠栳铌 怛铕钽 痤溧, 泐忸痂 蝾, 黩 潆 嚯屙桴 恹犷痤  铠栳赅 戾睃 牮栩屦 剜镨痤-妈腙, 潆 犷朦 牮栩屦 暑腱钽铕钼-鸯桊眍忄. 橡 铎 爨脲睃觇 恹犷痍圊 镳邃镱豚汔弪 戾睃 60 磬犭屙栝, 犷朦 犷朦 60. 溧眄铎 耠篦噱 狍溴 桉镱朦珙忄螯 怛铕铋 牮栩屦栝,  疋玎眍 蝈, 黩 麒耠 磬犭屙栝 潆 赅驿铋 桤 觐眈蝠箨鲨 珥圜栩咫 镳邂噱 60. 碾 恹犷痍 耦铗忮蝰蜮簋 镱矬鲨 耔眚弪梓羼觇扈 屐屙蜞扈 P-value 疣忭 0,062, 黩 恹 镱痤泐忸 铗戾蜿 箴钼 珥圜桁铖蜩 - 镳桧桁噱 汨镱蝈珞 眍痨嚯铎 疣耧疱溴脲龛. 碾 疣耱栩咫 屐屙蝾 蜞 驽 耥圜嚯 镳钼屦桦 磬 耦铗忮蝰蜮桢 眍痨嚯 疣耧疱溴脲龛 珥圜屙 忭篁痂 沭箫 钿桧嚓钼 镳铎铗铕铎. 绣珞朦蜞螓 囗嚯桤 镳邃耱噔脲睇 耠邃簋 蜞犭桷.

亦犭桷 6. 橡钼屦赅 磬 眍痨嚯铖螯 疣耧疱溴脲龛 忭篁痂 沭箫.

   

枢 忤漤 桤 蜞犭桷 6, 怦 疣耧疱溴脲龛 恹犷痤 铒疱溴脲眄 箴钼礤 珥圜桁铖蜩 (p0,05) 祛骓 聍栩囹 眍痨嚯. 袜桁屙簋 珥圜桁铖螯 桁邋 汨镱蝈玎 眍痨嚯铖蜩, 潆 疱珞朦蜞蝾 铒, 觐蝾痤 疱镱痱屦睇 徨腩 磬躅滂腭 镱 镳铎铗铕铎 ELIP. 疋玷 蝈, 黩 潆 铋 沭箫稃 珥圜桁铖螯 磬躅滂蝰 磬 镳邃咫 镳桧蝾泐 磬扈 箴钼 漕耱钼屦眍耱, 囗嚯桤 疣耧疱溴脲龛 猁 镳钼邃屙 蜞 驽 忭篁痂 镱溷痼镲, 赅 潆 恹铉磬麇眄铋 沭箫稃, 蜞 潆 铖蜞朦睇, 潆 镱恹龛 蝾黜铖蜩 恹忸漕.

橡钼邃屙睇 疣聍弪 镱赅玎腓, 黩 疣耧疱溴脲龛 忭篁痂 怦艴 12 镱溷痼镲 祛骓 麒蜞螯 眍痨嚯 箴钼礤 珥圜桁铖蜩 p0,15, 黩 怆弪 漕耱囹铟眍 恹耦觇 镱赅玎蝈脲. 碾 疣耱屙梃 耔眚弪梓羼觇扈 屐屙蜞扈, 眍痨嚯铖螯 忭篁痂 镱溷痼镲 镳钼屦屐 漯筱桁 蝈耱铎 - 牮栩屦桢 朽猷磬逆铋礤疣 (桎屙蜩麇 蝈耱 剜镨痤-予腙), .. 铎 耠篦噱 赅驿铋 铗溴朦眍 镱矬鲨 镱 24 疣耱屙. 羽钼屙 珥圜桁铖蜩 玟羼 忸 怦艴 耠篦 犷朦 0,100.

疱珞朦蜞蝈 铖簌羼蜮脲眄钽 囗嚯桤, 猁腩 镳桧蝾 疱龛 聍栩囹 疣耧疱溴脲龛 磬 溧眄 眍痨嚯 耦铗忮蝰蜮簋 溧朦礤轼屐 桉耠邃钼囗棹 戾蝾漕 ANOVA.

蔫耧屦耔铐睇 囗嚯桤 蝠遽箦 蜞赕, 黩钺 戾驿 觐祜脲犟囔 耦犭嚯铖 疣忮眈蜮 滂耧屦耔. 橡邃镱腩驽龛 钺 钿眍痤漤铖蜩 滂耧屦耔 疣珉梓睇 沭箫镟 怆弪 忄骓 潆 滂耧屦耔铐眍泐 囗嚯桤. 腓蝈疣蝮疱 镱 铎 忸镳铖 镳邃豚汔弪 ( 漕赅玎磬 镳噔铎铟眍耱 镳邃腩驽龛) 箐钼脲蜮铕螯 蜞觐 蝠遽钼囗桢 箴噔龛忄龛屐 麒耠 珥圜屙栝 赅驿铎 桤 觐祜脲犟钼, 黩 猁腩 皲咫囗 磬扈. 枢礓桎囹 磬 箐嚯屙桢 恹徼疣腓顸 耠篦嚅睇 钺疣珙 镳 镱祛 沐礤疣蝾疣 耠篦嚅睇 麒皴. 湾 耢铗 磬  潆 镳钼屦觇 翦牝桠眍耱 镳邃簌邈 汔, 桉镱朦珙忄腓 牮栩屦梃 隋忮磬 拎痱脲蝌.

署栩屦栝 隋忮磬 (钿眍痤漤铖蜩 滂耧屦耔): 碾 赅驿铋 玎忤耔祛 镥疱戾眄铋, 镳钼钿栩 滂耧屦耔铐睇 囗嚯桤 噌耦膻蝽 铗觌铐屙栝 磬犭噱禧 珥圜屙栝 铗 耦铗忮蝰蜮簋 耩邃龛 镱 沭箫镟. 篷腓 牮栩屦栝 隋忮磬 怆弪 耱囹桉蜩麇耜 珥圜桁, 汨镱蝈玎 钺 钿眍痤漤铖蜩 滂耧屦耔 漕腈磬 猁螯 铗忮疸眢蜞.

署栩屦栝 拎痱脲蝌 耱囹桉蜩麇耜栝 牮栩屦栝, 镱玮铍栝 镳钼屦螯 疣忮眈蜮 滂耧屦耔 礤耜铍桴 (溻篚 犷脲) 恹犷痤. 腕脲忄 汨镱蝈玎 镳邃镱豚汔弪, 黩 疣耨爨蝠桠噱禧 恹犷痍 镱塍麇睇 桤 沐礤疣朦睇 耦忸牦镯铖蝈, 钺豚溧桴 钿桧嚓钼 滂耧屦耔扈. 署栩屦栝 拎痱脲蝌 怆弪 镟疣戾蝠梓羼觇 铖眍忄 磬 漕镱腠栩咫铎 镳邃镱腩驽龛 眍痨嚯铖蜩 恹犷痤 溧眄. 项铎 镥疱 镳桁屙屙桢 牮栩屦 拎痱脲蝌 疱觐戾礓箦蝰 恹镱腠栩 镳钼屦牦 眍痨嚯铖蜩.

湾耧铖钺眍耱 箐钼脲蜮铕栩 溧眄铎 漕矬龛 镳桠邃蛤 犷朦 铖蜞蜿囔, 镱耜铍 铖蜞蝾  鬣耱 珥圜屙 溧眄, 觐蝾疣 礤 钺耥弪 翦牝囔 噤滂蜩忭钽 羿牝铕. 蒡 疋铪 铟屦邃 镳桠邃蛤 犷朦祗 耩邃礤祗 赈噤疣蝮 铖蜞蜿, 觐蝾瘥 溧耱 犷朦 耱囗溧痱睇 铠栳觇 耩邃龛 珥圜屙栝, 镱铎 镱蝠遽簋蝰 犭桢 疣珉梓 戾驿 耩邃龛扈 忮腓麒磬扈 羿牝铕钼, 黩钺 铐 猁腓 镳桤磬睇 珥圜桁.

橡钼屦牦 钿眍痤漤铖蜩 滂耧屦耔 镳钼钿桦 耥圜嚯 镳 耩噔礤龛 镱溷痼镲 腓龛 桎屙蜩黜 耧疱耨桀眄 赅耨弪囔 戾驿 耦犷 忭篁痂 沭箫稃 镳铎铗铕 (蜞犭. 7). 溧朦礤轼屐, 潆 犷朦 筲屦屙眍耱 钿眍痤漤铖蜩 滂耧屦耔, 耩噔龛忄腓 桴 潆 疣珥 腓龛, 忭篁痂 镱溷痼镲 (蜞犭. 8, 9).

亦犭桷 7. 橡钼屦赅 磬 钿眍痤漤铖螯 疣忮眈蜮 滂耧屦耔.

   

蝾 耠篦噱, 觐沅 珥圜屙桢 p-value 镳邂噱 箴钼屙 珥圜桁铖蜩 (潆 犷朦眈蜮 镱漕犴 桉耠邃钼囗栝 - 0,05), 滂耧屦耔 镳桧桁帼蝰 镳桧噤脲驵扈 钿眍 恹犷痍. 枢 忤漤 桤 蜞犭桷 8, 潆 镱矬鲨 耦溴疰帙桴 疣耱栩咫 屐屙螓 怦 蝠 恹犷痍 疣珥 疣耧铍铈屙桢 屐屙蝾 (觐眚痤朦, II I-IV), 徨 篦弪 怆龛 戾耱 怦蝠铋觇 耧疱耨桀眄铋 赅耨弪 漯筱桴, 礤 觐眚痤腓痼屐 磬扈 篑腩忤, 镳桧噤脲驵 钿眍 恹犷痍 忮顸爨 恹耦觇 箴钼礤 珥圜桁铖蜩. 碾 疣耱屙栝 耔眚弪梓羼觇扈 屐屙蜞扈 耔蝮圉 囗嚯钽梓磬. 橡 篦弪 怆龛 耠篦嚅睇 羿牝铕钼 牮栩屦栝 隋忤磬 怦 妁 泐忸痂 钺 钿眍痤漤铖蜩 滂耧屦耔. 义 礤 戾礤, 忤漤, 黩 镱 蝈耱 拎痱脲蜞 箴钼屙 珥圜桁铖蜩 钿眍痤漤铖蜩 滂耧屦耔 忭篁痂 腓龛, 礤觐蝾瘥 耠篦 龛驽 镱痤泐忸泐. 武磬觐 铗 蝈耱 铟屙 黧怦蜮栩咫屙 铗觌铐屙棹 铗 眍痨嚯铖蜩 恹犷痍, 蜞 黩 桁, 溧眄铎 耠篦噱, 镱玮铍弪 镳屙遽疱鼽. 蝾祗 驽, 忄骓 镱龛爨螯, 黩 镳邃镱腩驽龛 钿眍痤漤铖蜩 滂耧屦耔 礤 蜞 牮栩梓眍, 赅 漯筱桢 镳邃镱腩驽龛 滂耧屦耔铐眍泐 囗嚯桤, 鬣耱眍耱, 耠篦噱 襻嚯囗耔痤忄眄 镫囗钼 ( 疣忭 麒耠铎 磬犭屙栝 n, 赅 磬 耠篦噱). 令脲 蝾泐, 铒桉囗睇 龛驽 牮栩屦梃 襦扈 镱 皴徨 礤 钺玎蝈朦眍 怆 铟屙 篑蝾轺桠 (痤徉耱睇扈) (磬镳桁屦, 疣犷蝈 Glass and Hopkins, 1996, 耱. 436,  牮栩屦梃 磬琨忄 "羿蜞朦眍 礤漕疣犷蜞眄"; 耢. 蜞赕 铒桉囗桢 桴 牮栩屦桢). 蝾 怵屐 赅 襦爨 蝈蹴桕 滂耧屦耔铐眍泐 囗嚯桤 怆弪 "痤徉耱眍". 蒡铗 蝈痨桧, 桉镱朦珞屐 耱囹桉蜩赅扈, 铉磬鬣弪, 黩 溧眄 漕矬龛 祛泱 猁螯 礤觐蝾痤 耱屣屙 磬痼睇, 眍, 礤耢铗 磬 , 蝈蹴桕 祛骓 桉镱朦珙忄螯.

4.3.2 ANOVA

项耠 镳钼邃屙眍泐 囗嚯桤 溧眄 磬 镳噔铎铟眍耱 镳桁屙屙 龛 戾蝾溧 ANOVA, 猁腩 镳钼屦屙 礤镱耩邃耱忮眄 襦祛 怆龛 屐屙蝾 磬 耧疱耨棹 沐眍 (蜞犭. 10).

溧眄铎 耠篦噱 羿牝铕 1  溧眄 镱 觐眈蝠箨鲨, 铐 翳犟桊钼囗.

脏牝铕 2 镱 腓龛 忭篁痂 觐眈蝠箨鲨,  耠篦嚅睇 羿牝铕. 项怛铕屐铖螯 忭篁痂 腓龛 6 珥圜屙栝 ( 觌铐). 蠕 戾蝾溧 铖眍忄磬 磬 耩噔礤龛 忮腓麒 滂耧屦耔, 镱痤驿屙睇 怆龛屐 忭屮礤泐 羿牝铕 DA (耔耱屐囹梓羼赅, 桦 戾驺痼镲钼 滂耧屦耔) 滂耧屦耔 恹犷痍, 铖忸犷驿屙眍 铗 忸玟彘耱忤 忭屮礤泐 羿牝铕 DR (铖蜞蝾黜, 桦 忭篁痂沭箫镱忄 滂耧屦耔).

谚耱屐囹梓羼赅 滂耧屦耔 铒疱溴弪 赅 滂耧屦耔 戾驿 耩邃龛扈 赅驿铋 桤 k 沭箫, 耦铗忮蝰蜮簋 疣珉梓睇 箴钼 羿牝铕. 蒡 滂耧屦耔 踵疣牝屦桤箦 耱屣屙 疣玑痤襦 篑疱漤屙睇 镱 沭箫镟 珥圜屙栝, 邋 忮腓麒磬 玎忤耔 腓 铗 耱屣屙 怆龛 羿牝铕 磬 桤戾礤龛 耠篦嚅眍 忮腓麒睇.

   

务蜞蝾黜 滂耧屦耔 铒疱溴弪 赅 耦忸牦镯 忭篁痂沭箫镱忄 滂耧屦耔, 镳梓屐 赅驿铋 沭箫镥 磬犭噱禧 珥圜屙 忄瘘桊簋蝰 铗眍耔蝈朦眍 疋铄 沭箫镱忸 耩邃礤. 锐麒耠屙桢 铋 滂耧屦耔 桉觌噱 怆龛 疣耨爨蝠桠噱祛泐 羿牝铕, 邋 忮腓麒磬 铒疱溴弪 腓 镱沭屮眍耱 桤戾疱龛 怆龛屐 漯筱桴, 礤篦蝈眄 羿牝铕钼.

篷腓 耔耱屐囹梓羼赅 滂耧屦耔 耦桤戾痂爨 铖蜞蝾黜铋, 蝾 怆龛 羿牝铕 礤朦 镳桤磬螯 珥圜桁, 栳 钺耥屐铄 桁 疣珥铑狃噻桢 镱忮溴龛 耠篦嚅眍 忮腓麒睇 耦桤戾痂祛 疣珥铑狃噻桢, 镱痤驿屙睇 礤蝾黜铖螯 桤戾疱龛. 酿筱铄 溴腩, 羼腓 钺耥屙磬 磬腓麒屐 羿牝铕 鬣耱 滂耧屦耔 耋耱忮眄 犷朦, 麇 邋 鬣耱, 镱痤驿屙磬 耠篦嚅睇扈 镱戾踵扈. 绣龛 珥圜桁铖蜩 忸玟彘耱忤 忭屮礤泐 羿牝铕 镳桧桁噱蝰 磬 铖眍忄龛 耩噔礤龛 磬犭噱祛泐 蝈铕弪梓羼觐泐 珥圜屙栝 F-耱囹桉蜩觇 澡疣 秧邃尻铕.

蔓溻桡噱蝰 汨镱蝈玎 H0, 耦耱 蝾, 黩 疣耨爨蝠桠噱禧 羿牝铕 礤 铌噻噱 耋耱忮眄钽 怆龛 磬 桤戾礤龛 耠篦嚅眍 忮腓麒睇 (镱 耋耱怏, 汨镱蝈玎 钺 铗耋蝰蜮梃 耱囹桉蜩麇耜铋 疣珥桷 戾驿 沭箫镱恹扈 耩邃龛扈). 赅麇耱忮 耱囹桉蜩 恹徼疣 鬣耱睇, MS 戾驺 MS 戾骘钿沭 F1 F2 MS 戾骘钿沭 MS忭篁痂沭 扬疣忄 铗 F-铗眍龛 箨噻噱蝰 箴钼屙 珥圜桁铖蜩 箨噻囗眍 F耱囹桉蜩觇. 篷腓 珥圜桁铖螯 F-耱囹桉蜩觇 犭桤赅 眢膻, 羼螯 铖眍忄龛 镳桧螯 汨镱蝈珞 1 - 羿牝铕 珥圜桁 怆弪 磬 觐礤黜 疱珞朦蜞 (觐黻箴桊簋 H0 汨镱蝈玎). 溧眄铎 耠篦噱 珥圜桁铖螯 F1 潆 钺彖 觐眈蝠箨鲨 35S 潆 ELIP 戾睃 0.01 珥圜栩, 箴钼礤 珥圜桁铖蜩 0.01 潆 钺疣蝽钽 镳邃镱腩驽龛, 忮痦 汨镱蝈玎 珥圜桁铖蜩 羿牝铕钼 桴 溻篚 耠篦. 碾 RuBisCO 箴钼屙 珥圜桁铖蜩 镳嚓蜩麇耜 疣忮 眢膻, .. 忮痤蝽铖螯 铠栳铟眍 铗觌铐栩 眢脲怏 汨镱蝈珞 钺 铗耋蝰蜮梃 疣珉梓栝 镳嚓蜩麇耜 疣忭 眢膻, 黩 泐忸痂 耔朦眍 怆龛 屐屙蝾 磬 铗 镳铎铗铕. 潆 35Smin 箴钼屙 珥圜桁铖蜩 眢脲忸 汨镱蝈琨 泐疣玟 犷朦 0,05, 黩 镳桠钿栩 磬 恹忸潴 钺 铗耋蝰蜮梃 怆龛 桉镱朦珙忄眄 屐屙蝾 膻犷 桤 镱腩驽龛 磬 箴钼屙 耧疱耨梃 疱镱痱屦眍泐 徨腙 镱 桁 镳铎铗铕铎.

帖 祛驽 镳栝蜩 蜞觇 驽 玎觌屙, 镳桧螯 桦 铗觌铐栩 汨镱蝈珞 1, 铖眍恹忄顸 磬 蝾, 犷朦 桦 戾睃 珥圜屙桢 桁邋 F, 镱 耩噔礤龛 F牮栩 (珥圜屙桢 觐蝾痤泐 祛骓 忡螯 桤 蜞犭桷 潆 F疣耧疱溴脲龛 箴钼礤 珥圜桁铖蜩 0,05).

如 镱塍麇眄 溧眄 (蜞犭. 10) 祛骓 皲咫囹 恹忸 蝾, 黩, 徨珞耠钼眍, 磬栳铍栝 翦牝 怆龛 桉耠邃箦禧 屐屙蝾 磬犭噱蝰 潆 耔朦眍泐 疣耱栩咫钽 镳铎铗铕 RuBisCO 镳 桉镱朦珙忄龛 疣耱栩咫 疱泱蝾痦 屐屙蝾. 武磬觐 溧眄 囗嚯桤 礤 祛驽 耜噻囹 磬, 赅觐 桤 疣耧铍铈屙栝 屐屙蝾 怆弪 磬 箴钼屙 耧疱耨梃, 赅牦 耱铕铐. 碾 恹耥屙 桴 钺耱蝈朦耱 礤钺躅滂祛 镳钼羼蜩 溧朦礤轼桢 镱耱-躅 蝈耱.

褥邋 耢 箨噻囹 妁 漕忮痂蝈朦睇 桧蝈疴嚯 潆 疣珥铖蜩 耩邃龛, 溧栝 忸珈铈眍耱 耋滂螯 忮腓麒礤 疣珉梓栝. 令脲 蝾泐, 漕忮痂蝈朦睇 桧蝈疴嚯 怙铍礤 祛泱 玎戾龛螯 耱囹桉蜩麇耜桢 牮栩屦梃 镳 铞屙赍 耱囹桉蜩麇耜铋 珥圜桁铖蜩 疣珉梓栝. 腻腩 蝾, 黩 桉蜩眄 疣珥铖螯 耩邃龛 祛驽 磬躅滂螯 膻犷 蝾麝 漕忮痂蝈朦眍泐 桧蝈疴嚯. 项铎, 羼腓 镱塍麇眄 镳 疣犷蝈 恹犷痍囔 漕忮痂蝈朦睇 桧蝈疴嚯 耦溴疰栩 眢脲忸 珥圜屙桢,  珥圜栩, 黩 桉蜩眄 疣珥铖螯 耩邃龛 蜞赕 祛驽 猁螯 疣忭 眢膻. 央邃钼囹咫, 禧 礤 桁邋 铖眍忄龛 铗忮疸眢螯 眢脲怏 汨镱蝈珞. 疋铪 铟屦邃, 羼腓 漕忮痂蝈朦睇 桧蝈疴嚯 礤 耦溴疰栩 眢, 禧 祛驽 玎溧眄铋 筲屦屙眍耱 铗赅玎螯 铗 眢脲忸 汨镱蝈琨 聍栩囹 疣珉梓 耱囹桉蜩麇耜 珥圜桁 署栩屦栝 泥眄弪 耱痤栩 漕忮痂蝈朦睇 桧蝈疴嚯 潆 疣珥铖蜩 耩邃龛 赅驿钽 桤 铒钼, 耩噔龛忄 桴 觐眚痤脲.

碾 礤泐 禧 赅 疣 篦栩噱, 镳铛钿栩 腓 漕忮痂蝈朦睇 桧蝈疴嚯 潆 疣珥铖蜩 耩邃龛 麇疱 眢脲怏 疣珥铖螯. 篷腓 桧蝈疴嚯 镱镟溧弪 眢脲忄 疣珥铖螯,  珥圜栩, 黩 疣珥桷 戾驿 觐眚痤脲 铒铎 礤.

如 镱塍麇眄 溧眄 忤漤, 黩 磬 耧疱耨棹 镱 镳铎铗铕铎 RUBISCO 怆 钺 疣耧铍铈屙 屐屙蝾, 赅 钿眍, 蜞 钺彖 耱铕铐.

袜 ELIP 怆龛 铌噻噱蝰 腓 镳 綦囗觇痤忄龛 耧疱耨桀眄铋 赅耨弪 屐屙蜞扈 钺彖 耱铕铐 (蜞犭.11). 碾 35S 镳 95% 桧蝈疴嚯 疣珉梓栝 恹怆屙 礤 猁腩, 钿磬觐, 礤耢铗 磬 , 耦汶囫 溴轳蜮 蝈, 黩 疱珞朦蜞螓 滂耧屦耔铐眍泐 囗嚯桤 镱赅玎腓 磬腓麒 耋耱忮眄钽 疣珉梓, 禧 筲咫梓桦 漕忮痂蝈朦睇 桧蝈疴嚯 漕 90%.

项耠 钽 耱嚯 忤漤, 黩 潆 钽 耠篦 怆龛 铌噻噱 疣耧铍铈屙桢 屐屙蜞 腓 钿眍 耱铕铐. 耠篦噱 耔眚弪梓羼觇 屐屙蝾 潆 GeI-I 蜞 驽 猁 桉镱朦珙忄 90% 桧蝈疴嚯, .. 珥圜屙 潆 95% 腓 礤珥圜栩咫 镥疱牮嚯 眢脲怏 铗戾蜿 (蜞犭.12). 雾 铌噻帼 怆龛 磬 耧疱耨棹 35S 镳铎铗铕 忸 怦艴 耠篦 牮铎 GeII-II. 武磬觐 耩噻 忤漤, 黩 磬栳铍栝 翦牝 玎戾蝈 镳 疣耧铍铈屙梃 桴 镱耠邃钼囹咫铖蝈  镱 蝈麇龛 铗 耧疱耨桀眄铋 觐眈蝠箨鲨, 黩 镱潋忮疰溧弪 磬 镥疴梓睇 镳邃镱腩驽龛.

翌沅 赅 耱囹桉蜩麇耜桢 蝈耱 珥圜桁铖蜩 镱赅琨忄 磬 忮痤蝽铖螯 蝾泐, 黩 耧屦桁屙蜞朦睇 疱珞朦蜞螓 铗腓鬣 铗 耠篦嚅眍泐 爨蝈爨蜩麇耜钽 铈桎囗, 桤戾疱龛 忮腓麒睇 翦牝 镱赅琨忄 铗眍耔蝈朦睇 忮腓麒睇 耧屦桁屙蜞朦眍泐 忸玟彘耱忤, 泐忸 钺 邈 耔脲.

亦犭桷 11. 念忮痂蝈朦睇 桧蝈疴嚯 潆 疣珥铖蜩 耩邃龛. 泥眄 镱 耧疱耨梃 赅驿铎 耧屦桁屙蝈 耩噔礤龛 觐眚痤脲. LL-龛骓栝 腓扈; DM-疣珥铖螯 耩邃龛; UL-忮瘐龛 腓扈.

   

洛腓麒睇 翦牝钼 铖钺屙眍 忄骓, .. 铐 镱玮铍 磬 耩噔龛忄螯 疣珈屦 耧屦桁屙蜞朦眍泐 怆龛 礤耜铍桴 疣珥 铒圊. 躅 铗腓麒 戾驿 铒铎 觐眚痤脲 祛驽 猁螯 桤戾疱眍 镳铞屙蜞, 蜞觇 恹觌噤觇 鬣耱 耠铈眍 桉蝾腙钼囹 镳嚓蜩麇耜 礤忸珈铈眍 桉镱朦珙忄螯 潆 麇蜿铋 耥铋 桧蝈痫疱蜞鲨 疋弪 耧屦桁屙蜞朦睇 镟疣滂沆. 闰嚓, 禧 箸磬腓, 赅觐 疣耧铍铈屙桢 屐屙蝾 忸玟彘耱怏弪 磬 礤觐蝾瘥 桤 耧疱耨桀眄 赅耨弪. 篷蝈耱忮眄, 磬 玎桧蝈疱耦忄豚 忮腓麒磬 翦牝, 觐蝾痼 恹麒耠屙睇 磬扈 羿牝铕 铌噻帼 磬 箴钼屙 耧疱耨梃 桉镱朦珞屐 铒 徨腙钼. 朽珥桷 戾驿 铒铎 觐眚痤脲 怙铍礤 忸珈铈眍 镳钹磬腓玷痤忄螯 麇疱 铞屙牦 忮腓麒睇 翦牝 羿牝铕. 碾 钽 镳桁屙 耠邃簋 嚯泐痂蜢.

碾 磬鬣豚 祛骓 铞屙栩 忮腓麒眢 翦牝 潆 羿牝铕 鲥腩.

^ 亦犭桷 13. 脏牝铕 f. 项玮铍弪 铞屙栩 忮腓麒眢 翦牝 潆 羿牝铕 鲥腩. 0.1 耠噌 翦牝; 0.1-0.4 耩邃龛 翦牝; 0.4 耔朦睇 翦牝.

   

如 蜞犭桷 13 忤漤, 黩 磬栳铍栝 翦牝 羿牝铕 镳铊玮钿栩 磬 沐睇, 磬躅羼 镱 镳铎铗铕铎 Rubisco (0,25), 黧螯 戾礤 磬 35S (0,2), 妁 耠噌邋 ^ 翦牝 潆 ELIP (0,17). 碾 35Smin 珥圜屙桢 f 箨噻噱 磬 铟屙 耠噌 翦牝, 黩 泐忸痂 镳嚓蜩麇耜 镱腠铎 铗耋蝰蜮梃 怆龛 铎 耠篦噱 镱腠铖螯 ^ 耦汶囫箦蝰 疱珞朦蜞蜞扈 桢疣瘐梓羼觐泐 f 滂耧屦耔铐眍泐 囗嚯桤. 碾 35S 镳铎铗铕, 怆龛 疱泱蝾痦 屐屙蝾 忤痼耥钽 徉牝屦栲朦眍泐 镳铊聃铈溴龛 磬 耧疱耨棹 GUS 镳嚓蜩麇耜 桎屙蜩黜 蜞觐恹 潆 钽 驽 镳铎铗铕, 眍 疣耱栩咫 屐屙蜞扈 漯筱桁 疱镱痱屦睇 沐眍. 袜 铎 镳桁屦 忤漤, 黩 疣琊噌铗囗睇 磬扈 疣耱栩咫 疱泱蝾痦 邃桧桷 祛泱 铌噻囹 怆龛 耩噔龛祛 忤痼耥 屐屙蜞扈 , 蜞觇 钺疣珙, 镱潋忮疰溧 疋铪 翦牝桠眍耱.

碾 铞屙觇 怆龛 疣珥钽 疣耧铍铈屙 屐屙蝾 祛骓 桉镱朦珙忄螯 溴朦蝮 暑蹂磬 (铞屙桠噱 忮腓麒眢 翦牝 - 耔塍 怆龛 羿牝铕).

腻朦蜞 暑蹂磬 忮腓麒磬 翦牝, 桉镱朦珞屐 潆 钺磬痼驽龛 耱囗溧痱桤桊钼囗眍 疣珥桷 戾驿 溻箪 耩邃龛扈 ( 溧眄铎 耠篦噱 铒 觐眚痤). 蒡 钿桧 桤 磬栳铍邋 镱漉钿 耧铖钺钼 潆 耩噔礤龛 溻篚 耩邃龛. 溴朦螓 暑蹂磬 羼螯 礤耜铍 镳彖祗耱 磬 漯筱桁 戾蝾溧扈 桤戾疱龛 耔臌 翦牝. 钿眍 耱铕铐 疣耱簌 镱矬痦铖螯 钽 戾蝾溧 镱玮铍弪 镳桧桁囹 溴朦蝮 玎 耱囗溧痱桤桊钼囗眢 忮腓麒眢. 蜞觇 钺疣珙, 甯 镱漶麇螓 溴豚 忸珈铈睇 礤戾潆屙眍 耩噔礤龛 镱塍麇眄 珥圜屙栝 漯筱桁 桉耠邃钼囗扈. 漯筱铋 耱铕铐, 镳邃镱腩驽龛 蝾, 黩 忮腓麒睇 翦牝 戾礤 0.2 祛骓 聍栩囹 礤珥圜栩咫, 铌铍 0.5 耩邃龛扈, 镱耠

0.8 犷朦扈, 镱玮铍弪 磬 铞屙栩 桉耠邃箦禧 翦牝 镱 恹铒桉囗眍 嚯. 令脲 蝾泐, 溴朦蜞 镱赅琨忄弪 礤 蝾朦觐 耔塍 怆龛, 眍 邈 磬镳噔脲龛.

疹 猁腩 磬镨襦眍 桉麇痫帼 觐腓麇耱忸 耱囹彘 溴蜞腓玷痤忄眄 戾蝾溧 镱漶麇蜞 疣珈屦钼 翦牝, 镱 牮嚅礤 戾疱 潆 礤觐蝾瘥 徼铍钽梓羼觇 疣聍弪钼 , 鬣耱眍耱, 潆 祉钽桴 桉耠邃钼囹咫彘, 礤钺躅滂 犷脲 镳铖蝾 镱漉钿. 腻朦蝮 祛骓 镱聍栩囹 赅 疣珥桷 耩邃龛 磬 钺滂礤眄铄 耱囗溧痱眍 铗觌铐屙桢 (pooled standard deviation) (Thalheimer et al., 2002). 耋铖蜩, 忮腓麒磬 翦牝  羼螯 疣珥桷 戾驿 溻箪 耩邃龛扈 (磬镳桁屦 铒 扈眢 觐眚痤朦) 镱溴脲眄 磬 耱囗溧痱眍 铗觌铐屙桢 镳 疣珥 篑腩忤. 蒡 溴脲龛 磬 耱囗溧痱眍 铗觌铐屙桢 镱玮铍弪 磬 耩噔龛螯 忮腓麒睇 翦牝钼 怦邈 耧屦桁屙蜞.

X1 - X 2 d Cohen S pooled

   

如 溧眄 蜞犭桷 (蜞犭. 14 15) 禧 忤滂, 黩 磬栳铍邋 怆龛 铌噻噱 疣耧铍铈屙桢 疣耱栩咫 屐屙蝾 溻篚 耱铕铐 磬 耔朦睇 疣耱栩咫 镳铎铗铕 RuBisCO. 武磬觐 怆龛 铖蜞朦睇 耠篦 镱漕犴 镱耠邃钼囹咫铖扈, 篦栩 耱囗溧痱睇 镳桎屦骊, 蜞 驽 祛骓 聍栩囹 珥圜栩咫. 锐镱朦珙忄龛 耔眚弪梓羼觇 屐屙蝾 怆弪, 铖眍忭铎, 镳 桴 疣耧铍铈屙梃 腓 钿眍 耱铕铐 铗 耧疱耨桀眄铋 赅耨弪, 铖钺屙眍 耠篦噱 GeII. 填骓 铗戾蜩螯 蜞赕 礤觐蝾痤 怆龛 耠篦噱 GeI-I. 物螯 驽, 耩噻 忤漤, 黩 襦祛 翦牝桠眍 镱腩驽龛, 怆龛 耔眚弪梓羼觇 镱耠邃钼囹咫铖蝈 怙铍礤 耩噔龛祛 疣耱栩咫, 蜞觐 驽 铕桢眚圉梃. 碾 觐眈蝠箨鲨 GeII d(Cohen)0.8, 潆 疣耱栩咫 屐屙蝾 I-IV d(Cohen)0.7.

亦 驽 钿龛 桤 磬栳铍邋 鬣耱 怦蝠鬻帼躐 戾蝾漕 铒疱溴脲龛 耔臌 翦牝 潆 滂耧屦耔铐眍泐 囗嚯桤 怆弪 恹麒耠屙桢 2 (蒡 赈噤疣蝈). 2  漕 钺 滂耧屦耔 镳栾桉噱爨 翦牝. 蒡 怆弪 漕忸朦眍 礤钺牝桠眍 铞屙觐 镳 爨腩 觐腓麇耱忮 溧眄, 钿磬觐, 镱 戾疱 筲咫梓屙 桴 觐腓麇耱忄, 耢妁屙桢 铞屙觇 耥桄噱蝰.

   

蔓忸潲, 觐蝾瘥 祛骓 皲咫囹 桤 镱塍麇眄 疣聍弪钼 (蜞犭. 16 17) 鲥腩 耦汶囫簋蝰 溧眄, 镱塍麇眄 镳 镱漶麇蝈 溴朦螓 暑蹂磬. 武磬觐 溧眄铎 耠篦噱 耩噔龛忄螯 翦牝 怆龛 耔眚弪梓羼觇 疣耱栩咫 屐屙蝾 磬镳祗 礤 疱觐戾礓箦蝰, 疋玷 漕耱囹铟眍 耔朦睇 疣珉梓桢 疱镳彗屙蜞蜩忭铖蜩 恹犷痤.

软蝈疱耥 狍溴 蜞 驽 镳铖脲滂螯, 赅 戾弪 觐祜铐屙 滂耧屦耔 耠篦嚅睇 羿牝铕钼. 磬 耠篦噱  镳桧噤脲骓铖螯 桉耠邃箦祛泐 钺疣琏 铒疱溴脲眄铋 腓龛 觌铐, 镳 桉镱朦珙忄龛 疱泱蝾痦 屐屙蝾 (蜞犭.

18 19).

亦犭桷 18. 锣痂圉桀眄 觐祜铐屙蜞 箴钼礤 耧疱耨梃 35S-GUS 耔眚弪梓羼觇扈 屐屙蜞扈. VC 忄痂圉桀眄 觐祜铐屙蜞 ; NF-忄痂圉桀眄 觐祜铐屙蜞 戾驿 腓龛扈; RES-忄痂圉桀眄 觐祜铐屙蜞 戾驿 觌铐囔.

   

亦犭桷 19. 锣痂圉桀眄 觐祜铐屙蜞 箴钼礤 耧疱耨梃 RuBisCO-GFP, ELIP-GFP, 35S-GUS 35SminGUS 疣耱栩咫 屐屙蜞扈. NF-忄痂圉桀眄 觐祜铐屙蜞 戾驿 腓龛扈; RES-忄痂圉桀眄 觐祜铐屙蜞 戾驿 觌铐囔.

   

妈漤, 黩 疣耧疱溴脲龛 觐祜铐屙螓 忄痂圉梃 戾驿 礤翳犟桊钼囗睇扈 羿牝铕囔 潆 35S 徨 疱泱蝾痦 屐屙蝾 镳嚓蜩麇耜 桎屙蜩黜 耦 怛铕 铒铎 2013 (NF pBIG=56,8%, NF pBI35S=55.89%), 礤耢铗 磬 蝾, 黩 镳钼钿桦铖 桤戾疱龛 耧疱耨梃 溻篚 疣珥 徨腙钼, 溻箪 疣珥 戾蝾溧扈.

蒡 溧眄 漕镱腠栩咫, 躅 觐疋屙眍, 镱潋忮疰溧弪 麒耱铗 耧屦桁屙蝾 戾蝾滂觇 麒耠屙眍泐 囗嚯桤.

如 桴 蜞犭桷 蜞赕 忤漤, 黩 潆 耔朦睇 镳铎铗铕钼, 铖钺屙眍耱 潆 疣耱栩咫钽 Rubisco, 犷朦 耦耱噔 觐祜铐屙螓 滂耧屦耔 镳桴钿栩 磬 疣珉梓桢 溧眄 镱 铗溴朦睇 腓龛, 蝾沅 赅 潆 耠噌 铌铍 60% 镳桴钿栩 磬 戾骊腩磬朦眢 疣珥桷. 蒡 泐忸痂 蝾, 黩 潆 耔朦睇 镳铎铗铕钼 犷脲 忄骓 怆弪 戾耱 怦蝠噼忄龛 耧疱耨桀眄铋 赅耨弪, 甯 铌痼驽龛 觐镨轫铖螯, 镳梓屐 潆 疣耱栩咫钽 镳铎铗铕  篑腩忤 忸玟彘耱怏 犷朦 耱屣屙 ( 溧眄铎 耠篦噱 禧 耩噔龛忄屐 汶噔睇 钺疣珙 觐眚痤朦睇 镱矬鲨). 蝾 怵屐 赅 潆 耠噌 镳铎铗铕钼 犷朦 忄痂噌咫铖螯 镳桠眍耔 疣珉梓桢 箴钼礤 耧疱耨梃 戾驿 觌铐囔 钿眍 腓龛, 觐蝾疣 疋铪 铟屦邃 玎忤耔 铗 祉铈羼蜮 羿牝铕钼, 蜞觇 赅 铖忮眄铖螯, 忄瘘桊钼囗桢 耦溴疰囗 耦脲 扈牮铨脲戾眚钼 镱麾, 甯 怆噫眍耱, 磬腓麒 桦 铗耋蝰蜮桢 玎疣驽龛 (赅 忤痼襦扈, 蜞 怵邃栩咫扈) 铗溴朦睇 疣耱屙栝 .. 码龛 羿牝铕钼 踵疣牝屦睇 潆 铒疱溴脲眄 腓龛 潆 35Smin 耦耱噔弪 铌铍 40%, 潆 ELIP 蝾泐 戾睃

- 铌铍 30%. 妈滂祛, 镳 耧疱耨梃 磬 扈龛爨朦眍 箴钼礤, 怆龛 铌痼驽龛 礤 蜞 耔朦眍. 武磬觐 祛骓 铗戾蜩螯, 黩 躅 潆 觐眚痤朦眍 觐眈蝠箨鲨 耦溴疰帙彘 耔朦睇 疣耱栩咫 镳铎铗铕 Rubisco, 镳桧噤脲骓铖螯 铒疱溴脲眄铋 腓龛 怆弪 磬栳铍邋 忄骓 觐祜铐屙蝾 潆 箴钼 耧疱耨梃 疱镱痱屦眍泐 徨腙, 镳 疣耧铍铈屙梃 桉镱朦珞屐 磬扈 屐屙蝾 溻篚 耱铕铐 铗 耧疱耨桀眄铋 赅耨弪 镱玮铍弪 溻 疣玎 耥桤栩 箴钼屙 忄痂噌咫铖蜩 戾驿 腓龛扈, 珥圜栩 耱噌桦桤桊钼囹 耧疱耨棹 镱 桁 镳铎铗铕铎, 溴豚 邋 戾礤 玎忤耔祛 铗 蝾麝 桧皴瘀梃 觐眈蝠箨鲨. 碾 镳铎铗铕 ELIP 蜞觐 怆龛 礤珥圜栩咫, 黩, 忤滂祛, 疋玎眍 蝈, 黩 铎 耠篦噱 怆龛 羿牝铕钼 镳桧噤脲骓铖螯 腓龛 桤磬鬣朦眍 桁邋 龛珀铄 珥圜屙桢. 袜 忤痼耥 镳铎铗铕 蜞觐泐 怆龛 镳桁屙屙睇 屐屙螓 礤 铌噻帼.

   

1 谚眚弪梓羼觇 屐屙螓, 耦玟囗睇 磬 铖眍忮 蝈痨桧囹铕钼 沐眍 嚆痤徉牝屦栲朦眍泐 nos 忤痼耥钽 35S CaMV 镳铊聃铈溴龛 篑桦桠帼 耧疱耨棹 沐蝈痤腩汨黜 沐眍 疣耱屙 镱 耔朦睇 忤痼耥 镳铎铗铕铎 35S CaMV 镳 腩赅腓玎鲨 镥疱 镳铎铗铕铎.

2 项赅玎眍, 黩 疣耧铍铈屙桢 疣耱栩咫 耐-屐屙蝾 蝈痨桧桊簋 蝠囗耜痂秭棹 (ATs), 镳 膻犷 疣耧铍铈屙梃 铗眍耔蝈朦眍 赅耨弪 耧疱耨梃 铌噻噱 犷脲 耔朦眍 怆龛 磬 耧疱耨棹 沐眍 镱 疣耱栩咫 镳铎铗铕囔 镱 耩噔礤龛 忤痼耥. 逾咫梓屙桢 箴钼 耧疱耨梃 镳祛 觐痧咫桊箦 桦铋

镳铎铗铕 赅 疣耱栩咫钽, 蜞 忤痼耥钽 镳铊聃铈溴龛.

3 项赅玎眍, 黩 疣耧铍铈屙桢 耐-屐屙蝾 赅 钿眍, 蜞 钺彖 耱铕铐 耧疱耨桀眄铋 赅耨弪 篑桦桠噱 耧疱耨棹 沐眍 耔朦眍泐 疣耱栩咫钽 镳铎铗铕 RuBisCO. 蓐耧疱耨棹 耔朦眍泐 忤痼耥钽 镳铎铗铕 35S CaMV 篑桦桠帼 耐-屐屙螓 疣耧铍铈屙睇 蝾朦觐 5'-觐眦 沐磬. 袜 耠噌 镳铎铗铕 35S min ELIP 怆龛 屐屙蝾 戾礤 恹疣驽眍.

4 玉蜞眍怆屙, 黩 疣耱栩咫 耐-屐屙螓, 蝈痨桧桊簋 蝠囗耜痂秭棹 (ATs), 镳 疣珈妁屙梃 钺彖 耱铕铐 耧疱耨桀眄铋 赅耨弪 耥桄帼 镱玷鲨铐睇 翦牝 翦牝 觐镨轫铖蜩 漕 溻篚 疣 镳 耧疱耨梃, 镱 觐眚痤脲 耔朦眍泐 疣耱栩咫钽 镳铎铗铕 RuBisCO.

棱蝾 恹疣驵弪 犭嚆钿囵眍耱 耦蝠箐龛赅 耱囗鲨 桉牦耨蜮屙眍泐 觌桁囹 桀蝠铐 翳腓嚯 攘 欣 玎 镱祛 镳钼邃屙梃 桉耠邃钼囗栝 玎 祉钽铟桉脲眄 鲥眄 耦忮螓.

棱蝾 恹疣驵弪 犭嚆钿囵眍耱 觐腚尻蜩怏 豚犷疣蝾痂 徼铊眙铕爨蜩觇 软耱栩篁 爨蝈爨蜩麇耜桴 镳钺脲 徼铍钽梃 欣, 鬣耱眍耱 袜玷镱忸 袜翳皴 袜桦钼礤 玎 镱祛 镳钼邃屙梃 爨蝈爨蜩麇耜铋 钺疣犷蜿 疱珞朦蜞蝾.

6. 扬桉铌 腓蝈疣蝮瘥

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